spiber spiber

Biomolecules|April 12, 2021

Xanthurenic Acid Is the Main Pigment of Trichonephila clavata Gold Dragline Silk

Authors: Masayuki Fujiwara1,2, Nobuaki Kono1,2, Akiyoshi Hirayama1,2, Ali D. Malay3, Hiroyuki Nakamura4, Rintaro Ohtoshi4, Keiji Numata3, Masaru Tomita1,2,5 & Kazuharu Arakawa1,2,4*

DOI:110.3390/biom11040563

  • 1. Institute for Advanced Biosciences, Keio University
  • 2. Systems Biology Program, Graduate School of Media and Governance, Keio University
  • 3. Biomacromolecules Research Team, RIKEN Center for Sustainable Resource Science
  • 4. Spiber Inc.
  • 5. Faculty of Environment and Information Studies, Keio University

From "From "Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk" license approved by copyright holders.


Abstract

Spider silk is a natural fiber with remarkable strength, toughness, and elasticity that is attracting attention as a biomaterial of the future. Golden orb-weaving spiders (Trichonephila clavata) construct large, strong webs using golden threads. To characterize the pigment of golden T. clavata dragline silk, we used liquid chromatography and mass spectrometric analysis. We found that the major pigment in the golden dragline silk of T. clavata was xanthurenic acid. To investigate the possible function of the pigment, we tested the effect of xanthurenic acid on bacterial growth using gram-negative Escherichia coli and gram-positive Bacillus subtilis. We found that xanthurenic acid had a slight antibacterial effect. Furthermore, to investigate the UV tolerance of the T. clavata threads bleached of their golden color, we conducted tensile deformation tests and scanning electron microscope observations. However, in these experiments, no significant effect was observed. We therefore speculate that golden orb-weaving spiders use the pigment for other purposes, such as to attract their prey in the sunlight.

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本研究の遂行に際し、当社は 内閣府 革新的研究開発推進プログラム(ImPACT)「超高機能構造タンパク質による素材産業革命」 の助成を受けました。

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Proceedings of the National Academy of Science of the United States of America | August 6, 2021
Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk